Introduction

An ultra-low temperature freezer, commonly referred to as an ult freezer, is a freezer designed for the storage of the samples at the ultra-low temperatures, typically at -86 degrees Celsius, and it is used in the laboratories, the biobanks, the hospitals, and the research institutions for the long-term storage of the biological samples. The ultra-low temperature freezer consists of an insulated cabinet with the multiple doors, a cascade refrigeration system with the two or more compressors, a temperature control system, a temperature monitoring and an alarm system, and the shelves, and it maintains the temperature at the ultra-low level, with a high stability and uniformity, with features such as the digital temperature control, the temperature alarms, the data logging, the lock, and the backup systems; the ultra-low temperature freezer is used for the storage of the cells, the tissues, the DNA and the RNA, the proteins, the sera, the vaccines, the enzymes, and the other biological samples, for the long-term preservation, for the storage of the samples for the research and the diagnostics, and for many other applications, and it is available in various sizes, from the under-counter and the bench-top models to the large upright and the chest models; the ultra-low temperature freezer is an essential equipment for the cryogenic storage of the samples. Ultra-low temperature freezers are used in the laboratories, in the biobanks, in the hospitals, in the pharmaceutical industry, and for many other applications. With their ultra-low temperatures, ultra-low temperature freezers are essential for the long-term sample storage.

-86°C Under Counter ULT Freezer LUUF-T2010

-86°C Under Counter ULT Freezer LUUF-T2010



-86°C Under Counter Freezer consistent temperature control from -40°C to -86°C. It offers a 90 temperatures. The freezer includes multiple alarm systems, stainless steel interior, and...
-86⁰C Under Counter ULT Freezer LUUF-T2010

-86⁰C Under Counter ULT Freezer LUUF-T2010



-86⁰C Under Counter Freezer a high-performance storage unit with a 108L capacity, designed for the storage of large volume samples. It features a mixed refrigerant cooling system to mai...

Standards

IEC 61010-2-011:2019-Particular requirements for refrigerating equipment

IEC 62552-1:2015-Household refrigerating appliances-Characteristics and test methods-Part 1: General requirements

ISO 20387:2018-Biotechnology-Biobanking-General requirements for biobanking

ISO/TS 20388:2021-Biotechnology-Biobanking-Requirements for animal biological material

Complies with the above standards, but is not limited to them.

Features

1. Under-counter -86 degrees C ultra-low temperature freezer for space-saving storage of biological samples.

2. Compact upright freezer fitting under standard lab bench with -86 degrees C ultra-low temperature capability.

3. Suitable for research labs, clinical labs, biobanks, pharmaceutical, university and small laboratory settings.

4. Includes freezer body, compressor system, temperature controller, shelves, door, alarm and operation manual.

5. Supports storage of DNA, RNA, proteins, cells, reagents, biological samples and temperature-sensitive materials at -86 degrees C.

FAQ

Q1: What testing scenarios is this freezer mainly applied to?

A: The under-counter -86 degrees C ultra-low temperature freezer is used in research laboratories, clinical laboratories, biobanks, pharmaceutical facilities, universities, and small laboratory settings to provide compact, space-saving ultra-low temperature (-86 degrees C) storage for biological samples and reagents, fitting under a standard laboratory bench to save floor space while maintaining the deep cold required for long-term sample integrity. It supports molecular biology storage (DNA, RNA (genomic DNA, plasmids, PCR products, total RNA, mRNA - -80 degrees C prevents nuclease degradation and maintains integrity long-term), proteins (purified proteins, antibodies, enzymes, lysates, protein arrays - -80 prevents denaturation and degradation), cell and tissue storage (cell pellets, tissue samples (snap-frozen biopsy, surgical specimens), cell lines (in cryovials with DMSO, though liquid nitrogen is preferred for viable cells), biological samples (blood, serum, plasma, urine, CSF, saliva - long-term storage for clinical research/biobanking), reagents and kits (enzymes (restriction enzymes, polymerases, ligases - many shipped/stored at -20 but -80 for long-term), antibodies (long-term stock), molecular biology kits, primers/probes (lyophilized or in solution), pharmaceutical and biotech (API stocks, biologic drugs (monoclonal antibodies, vaccines, gene therapies - some require -80 storage), stability samples, investigational drugs), clinical/biobank (patient samples for retrospective studies, biomarker analysis, genomic/proteomic research - biobanking standard is -80 or liquid nitrogen vapor phase), and small labs where floor space is limited (under-counter design fits under bench, convenient access, smaller capacity (50-150 L) for moderate sample volumes).

Q2: What standards does the freezer comply with?

A: The under-counter -86 degrees C ultra-low freezer follows relevant IEC, ISO, WHO and GB/T standards including IEC 60601-1 (medical electrical equipment safety, if medical device), IEC 61010 (laboratory electrical equipment), ISO 15189 (medical laboratory quality), WHO guidelines for biological sample storage, and applicable ultra-low freezer standards. For vaccine/biologic storage, follow manufacturer storage requirements and pharmacopeia (USP, EP, ChP). The freezer should have temperature monitoring with alarm, and calibration should use certified -80 degree data logger/thermometer traceable to national standards. For pharmaceutical/GMP use, follow GMP (IQ/OQ/PQ, temperature mapping, 21 CFR Part 11 if electronic records). For clinical/biobank, follow ISBER (International Society for Biological and Environmental Repositories) best practices and ISO 9001/17025.

Q3: What is the warranty period for the whole freezer and key components?

A: The whole under-counter -86 freezer is covered by a 12-24 month warranty (ultra-low freezers often have 2-year warranty; compressors may have 5-year warranty). The cabinet, refrigeration system (compressors - cascade or Stirling, condenser, evaporator), temperature controller, shelves, door, door seal, and housing are covered, while door seals/gaskets (wear part), shelves, temperature sensors, batteries (alarm backup), and filters (if any) are consumables/accessories. Damage from improper use, power outage without backup (samples warm, compressor strain), door left open (temperature excursion, frost buildup, compressor overload), overloading (blocking air vents - poor temperature uniformity), dust-clogged condenser (compressor overheating/failure), using in high ambient temperature (>30-32 degrees C - compressor overload), or unauthorized repair is excluded. Extended warranty and service contracts (preventive maintenance, calibration, emergency response) are strongly recommended for -80 freezers (sample loss is costly). Compressors last 8-15 years with proper maintenance; door seals 3-5 years.

Q4: Is this device difficult to operate? Is professional training required?

A: Operation is simple for basic use (set temperature, load samples, monitor). No professional training required for basic operation, but for sample storage, staff should understand -80 storage protocols and emergency procedures. We provide operation manuals. Proper installation (place under stable bench or on floor in temperature-controlled room (18-25 degrees C ambient - avoid direct sunlight, heat sources, vents; ensure adequate clearance around unit for ventilation (compressor dissipates heat - under-counter models need rear/side clearance; do not enclose tightly), connect power (dedicated circuit, preferably with backup power/UPS or generator - power outages at -80 are critical; -80 freezers draw high power on startup and during operation), allow temperature stabilization (typically 24-48 hours before loading - verify temperature reaches -80 and stabilizes with calibrated logger before storing samples; ultra-low freezers take longer to cool down than standard freezers), temperature setting (set to -80 degrees C (or -70 to -86 per requirement; do not set colder than needed (increases energy use and compressor strain), sample loading (do not overload (leave space for air circulation - blocked airflow causes warm spots and compressor strain; load samples gradually (do not put large room-temperature batches at once - causes temperature spike; freeze samples in smaller batches or pre-chill), store samples in appropriate containers (cryovials, microcentrifuge tubes, 96-well plates - use -80-rated plastic (PP, PC); do not use glass (breaks at -80 due to thermal shock unless specially rated); seal tubes tightly (prevent evaporation, contamination, frost buildup), organize samples (boxes, racks, inventory system - -80 freezers should not be opened frequently (each opening raises temperature; minimize door open time; know sample location before opening), monitoring (check temperature at least daily (or continuous data logging - recommended for -80; use calibrated -80 data logger with min/max display and alarm; record temperatures), respond to temperature excursions immediately (if temp rises above -70 or -60: assess sample viability (some samples tolerate brief excursions, others do not - consult sample type/SOP; document excursion; if prolonged, transfer samples to backup freezer; do not discard without assessment), maintenance (regular cleaning (monthly: wipe exterior, check door seal, remove expired samples; do not defrost -80 freezers (they are auto-defrost or frost-free? Actually most -80 freezers are NOT auto-defrost (frost buildup is normal; manual defrost if excessive (transfer samples to backup, turn off, clean); clean condenser coils every 3-6 months (dust buildup reduces efficiency, causes compressor failure - vacuum/brush coils; critical for under-counter models with limited ventilation), door seal inspection (clean, check for cracks/wear - poor seal causes frost, temperature fluctuation, energy waste; replace if damaged), temperature calibration (every 6-12 months with certified -80 logger), backup power (test UPS/generator periodically - critical for -80 storage), troubleshooting (temperature too high (door left open, overloaded, vents blocked, condenser dirty, compressor failing, room too hot, power issue - check and address; if persists, transfer samples and call service), excessive frost (door seal leak, frequent door opening, humid environment, warm samples loaded - check seal, reduce openings, pre-freeze samples), alarm sounding (temperature excursion, door ajar, power failure - check immediately, document, respond), compressor running constantly (overloaded, dirty condenser, poor seal, room too hot - address), unusual noise (compressor, fan, vibration - service if persists), and safety (sample loss risk (-80 freezers are critical equipment - have backup plan: backup freezer, alarm notification (phone/email), emergency contact, sample inventory; consider -80 freezer with CO2 backup (some models have liquid CO2 backup for power failure)), electrical hazard (high-wattage compressor (500-1000W; dedicated circuit, proper grounding; do not use extension cords), cold burn hazard (-80 degrees C can cause frostbite/cold burns on contact - use cryogloves when handling samples/racks; do not touch interior surfaces with bare skin; use tools for sample retrieval), do not store flammable/volatile materials in -80 (fire/explosion hazard; use explosion-proof freezer if needed), do not store food/drinks (contamination, regulatory prohibition), ergonomics (under-counter model requires bending to access bottom shelves - use step stool, organize frequently used samples on upper shelves) are important for safe, reliable -80 storage.

Q5: What is the typical lead-time for order delivery?

A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.

Q6: Is regular calibration required? What is the recommended calibration cycle?

A: Temperature calibration recommended every 6-12 months using certified -80 degrees C data logger/thermometer (traceable to NIST/PTB/NIM; verify at multiple locations (center, top, bottom, door, walls - temperature mapping; typical uniformity +/- 3 to 5 degrees C for -80 freezers; under-counter models may have wider variation due to smaller size). For biobank/pharmaceutical use, temperature mapping (IQ/OQ) at installation and annually (or after relocation/repair). Routine temperature monitoring: daily (manual log) or continuous (data logger) - required for sample storage. Alarm testing (high/low temp, door ajar, power failure) every 3-6 months. Full performance verification annually (temperature accuracy, uniformity, recovery after door opening, alarm function, power consumption). For GMP/regulated use, follow applicable calibration schedules and maintain records (calibration certificates, temperature logs, excursion reports, maintenance records). -80 freezers are critical sample storage equipment - regular maintenance and calibration prevent sample loss. Condenser cleaning every 3-6 months is essential (dust is the #1 cause of -80 freezer compressor failure).

Q7: Are supporting consumables universal?

A: Temperature data loggers (certified -80 rated, NIST-traceable - available from lab supply; some are unit-specific (built-in), but standalone loggers are universal), door seals/gaskets (replacement - specific to model), shelves/racks (additional/replacement - specific to model), cryoboxes/racks (for sample organization - standard sizes (2-inch, 3-inch boxes; 96-well plate racks; universal or specific), cryogloves (for handling -80 samples - universal), batteries (for alarm backup/data logger - standard), cleaning supplies (mild detergent, disinfectant), and condenser cleaning tools (brush, vacuum) are available from laboratory supply companies, though door seals, shelves, and built-in sensors are unit-specific. Long-term consumable cost is minimal (occasional seal/shelf replacement, data logger calibration/battery). Energy cost is significant (-80 freezers consume 5-15 kWh/day - consider energy-efficient models).

Q8: Can I customize parameters or supporting accessories?

A: Customization includes capacity (small under-counter (50-100 L) for limited samples, medium (100-200 L) for general use - under-counter typically max ~150-200 L; for larger needs use upright floor model), temperature range (-86 degrees C (standard for biological samples), -50 to -86 adjustable, -40 to -86, -105 to -150 (cryogenic-freezer hybrid for some applications)), compressor type (cascade (two-stage refrigeration - traditional, reliable, but higher energy use, more heat) vs Stirling (single-stage, more energy-efficient, quieter, lighter - newer technology; both achieve -86), cooling system (forced air (better uniformity, faster recovery) vs static (simpler, less drying)), control (analog (dial, simple) or digital (LED display of actual temp, min/max recording, high/low alarms, set point lockout, password protection, data logging (USB, WiFi/Ethernet cloud monitoring, 21 CFR Part 11 for pharma)), backup (battery backup for alarm/monitoring (maintains monitoring during power outage), CO2/LN2 backup system (automatically injects liquid CO2 or LN2 to maintain temperature during power outage or compressor failure - critical for valuable samples; adds cost but prevents sample loss), construction (stainless steel interior (304 - corrosion-resistant, easy to clean), powder-coated steel exterior, insulated walls (foamed-in-place polyurethane - 75-100 mm for -80), self-closing door, door lock (key or combination - for sample security), casters (lockable, for mobility - under-counter models may have adjustable feet instead), safety/alarms (high/low temperature alarm, door ajar alarm, power failure alarm, remote alarm dialer (phone/email/SMS), battery backup, temperature chart recorder, certifications (FDA, CE, NMPA, ISO 13485, EN 60601, energy efficiency), accessories (cryoboxes, racks, data loggers, CO2 backup, temperature mapping service, calibration certificate (ISO 17025), language and accessories. Additional shelves, boxes, and accessories can be ordered separately.

Q9: Does the purchase include on-site installation and commissioning service?

A: Standard delivery includes under-counter -80 freezer, shelves, power cord, and instruction manual. On-site installation, temperature mapping, and training are not included for standard orders but are strongly recommended for -80 freezers (especially for GMP/pharmaceutical/biobank use - these are critical storage units requiring professional installation, power verification, temperature stabilization, and user training on emergency procedures; can be arranged at additional cost). For self-installation: place under bench, plug in (dedicated circuit), allow 24-48 hours to cool, verify temperature with calibrated logger, load samples gradually. Always have backup plan before storing valuable samples.

Q10: What types of samples are compatible with this equipment?

A: The under-counter -80 degrees C ultra-low freezer stores various biological and chemical samples requiring deep cold storage including nucleic acids (DNA (genomic, plasmid, PCR products, cDNA - stable for years at -80), RNA (total RNA, mRNA, miRNA - -80 prevents RNase degradation; use RNase-free tubes, stabilize with RNA later if needed), oligonucleotides (primers, probes - lyophilized or in TE buffer)), proteins and enzymes (purified proteins (recombinant, native - -80 prevents denaturation/aggregation; add glycerol (10-50%) for stability), antibodies (long-term stock aliquots - avoid repeated freeze-thaw; store working aliquots at -20 or 4), enzymes (restriction enzymes, polymerases, ligases, kinases - many are stored at -20 with glycerol; -80 for long-term stock), protein lysates, peptides), cells and tissues (cell pellets (harvested cells for protein/RNA extraction - snap freeze in liquid nitrogen then store at -80), tissue samples (snap-frozen biopsy, surgical, animal tissue - for RNA/protein/histology; use cryovials or foil), cell lines (in cryovials with DMSO (10%) - -80 is acceptable for short-term (months) but liquid nitrogen (-196) or vapor phase (-150 to -190) is preferred for long-term viable cell storage (cells degrade faster at -80 than at LN2)), biological fluids (blood (whole blood, serum, plasma - for biomarker, DNA, proteomics; separate serum/plasma before freezing), urine, CSF, saliva, semen, milk - store in aliquots to avoid freeze-thaw), reagents and kits (molecular biology kits (store components per manufacturer - some at -80), primers/probes, master mixes, enzymes, antibodies, lab-made reagents (aliquot and freeze)), pharmaceutical and biologics (API (active pharmaceutical ingredient) stocks, biologic drugs (monoclonal antibodies, vaccines (some mRNA vaccines require -80 or -20), gene therapies, cell therapies - follow manufacturer storage requirements), stability study samples (for ICH stability testing - long-term, accelerated, stress conditions), investigational drugs), and other temperature-sensitive materials (bacterial/viral stocks (glycerol stocks at -80 for long-term strain storage - standard method), fungal strains, plant seeds/tissue, environmental samples (soil, water - for DNA/RNA analysis), chemical standards (some unstable compounds)). NOT compatible with: Viable cell long-term storage (use liquid nitrogen (-196) or vapor phase (-150) for years of storage; -80 is only for months), samples requiring temperatures above -80 (use -20 freezer or refrigerator), flammable/explosive materials (use explosion-proof freezer), food/drinks (contamination), glass containers without -80 rating (thermal shock causes breakage - use plastic cryovials/tubes), and large sample volumes beyond capacity (under-counter models are limited (50-150 L); use floor model for biobank-scale storage). IMPORTANT: -80 degrees C ultra-low freezer fundamentals: (1) Why -80? Biological molecules (DNA, RNA, proteins) degrade via enzymatic and chemical reactions. At -80 degrees C, molecular motion is drastically reduced, effectively stopping most degradation reactions. -80 is the standard for long-term storage of nucleic acids, proteins, and non-viable biological samples. For viable cells, -80 is short-term (months); liquid nitrogen (-196) is long-term (years) because at -80, ice crystal formation and recrystallization can damage cells over time, and some enzymatic activity persists. (2) How -80 freezers work: Most -80 freezers use cascade refrigeration: two separate refrigeration systems (stages) with different refrigerants. The first stage (high temp) cools to ~-30 to -40 degrees C using a standard refrigerant (e.g., R-404A). The second stage (low temp) uses a different refrigerant (e.g., R-508B, a mixture) that can only work when pre-cooled by the first stage, achieving -80 to -86 degrees C. Newer Stirling cycle freezers use a single-stage Stirling engine with helium as working fluid - more energy efficient, lighter, quieter, but newer technology. (3) Critical considerations: - Warm-up rate: A -80 freezer, if power fails and door stays closed, may take 12-48+ hours to warm to -50 (depending on load, insulation, ambient temp). But if door is opened frequently or samples are warm, it warms faster. Backup power and CO2/LN2 backup are essential for valuable samples. - Energy use: -80 freezers are energy-intensive (5-15 kWh/day, comparable to a small house). Energy-efficient models (inverter compressors, better insulation) reduce cost. Setting to -70 instead of -80 (if samples tolerate) saves ~30% energy. - Condenser maintenance: Dust on condenser coils is the #1 cause of -80 freezer failure. Clean coils every 3-6 months. Under-counter models have limited ventilation - ensure clearance. - Temperature uniformity: -80 freezers have temperature gradients (door is warmest, bottom/coldest may vary). Use calibrated logger, map temperatures, store valuable samples away from door and walls. - Door opening: Each door opening raises interior temperature significantly (can rise 5-15 degrees C depending on duration). Minimize openings, know sample location, use organized inventory. - Frost buildup: -80 freezers accumulate frost from humid air entering during door openings. Excessive frost insulates evaporator, reduces efficiency. Most -80 freezers are manual defrost (auto-defrost would warm samples). Defrost if frost >1 cm (transfer samples to backup, turn off, clean). (4) Sample best practices: - Aliquot samples: Store in small aliquots to avoid repeated freeze-thaw (each freeze-thaw cycle degrades proteins/RNA). - Use appropriate tubes: Polypropylene (PP) cryovials rated for -80 (and LN2 if needed). Do not use regular microcentrifuge tubes for long-term -80 (some become brittle). Seal tightly (screw-cap with O-ring for cryovials). - Snap freeze: For tissue/cells, snap freeze in liquid nitrogen first (prevents ice crystal formation), then transfer to -80. - Inventory: Maintain sample inventory (location, date, contents). - Backup: Have backup freezer, alarm with remote notification, emergency contact list, sample priority list (what to save first if freezer fails). - Temperature monitoring: Continuous data logging with alarm is strongly recommended (not just daily manual checks). (5) Under-counter vs floor model: Under-counter -80 freezers are smaller (50-150 L), fit under bench, convenient for moderate sample volumes, but have less capacity and may have wider temperature variation. Floor models (300-800+ L) are for biobanks/high volume, better uniformity, more features. Choose based on sample volume and space. (6) Common errors: - Not cleaning condenser (compressor failure, sample loss). - No backup power/alarm (power outage = sample loss). - Overloading (warm spots, compressor strain). - Frequent door opening (temperature fluctuation, frost). - Storing viable cells long-term at -80 (use LN2). - Using glass tubes (breakage). - Not aliquoting (repeated freeze-thaw degrades samples). - Setting colder than needed (energy waste). - Ignoring temperature alarms (excursion damages samples). - Storing food/drinks (contamination, regulatory violation).

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