Introduction
A fluorescence analyzer is an instrument used to measure the fluorescence emitted by samples after excitation with light, providing information about the concentration of fluorescent substances, the activity of enzymes, the binding of molecules, and many other properties, and it is used in biochemistry, molecular biology, clinical diagnostics, environmental analysis, and many other fields. The analyzer excites the sample with light at an appropriate wavelength, using a lamp, laser, or LED, and measures the fluorescent light emitted by the sample at a longer wavelength with a detector, such as a photomultiplier tube or a CCD camera; the intensity of the fluorescence is related to the concentration of the fluorescent analyte, and the instrument can measure fluorescence in cuvettes, microplates, and other formats, and it can perform fluorescence intensity measurements, fluorescence polarization, time-resolved fluorescence, and other fluorescence techniques, and fluorescence analyzers are highly sensitive, allowing the detection of very low concentrations. Fluorescence analyzers are used for quantifying nucleic acids and proteins with fluorescent dyes, for measuring enzyme activity with fluorogenic substrates, for detecting and quantifying analytes in immunoassays, for DNA and RNA analysis, for environmental monitoring of pollutants, for water quality analysis, for cell analysis, for drug discovery screening, and for many other applications, and they are available as benchtop fluorometers, microplate readers, and imaging systems, with features such as multiple excitation and emission wavelengths, high sensitivity, and data analysis software. With their sensitivity, fluorescence analyzers are essential for fluorescence-based analysis.
Fluorescence Analyzer LFMA-T2010
Fluorescence Analyzer can detect across a range of 200 to 1100 nm, depending on the grating. It is equipped with advanced a highly sensitive spectrometer to detect weak fluorescence sign...
Fluorescence Analyzer LFMA-T2010
Fluorescence Analyzer is a modular designed instrument offering detection limit of 0.001 ng/ml. Features high sensitivity with high baseline stability, provides excellent short term and ...
Standards
JIS K 0115:2004-General rules for molecular absorptiometric analysis
ASTM E579-20-Standard Test Method for Limit of Detection and Limit of Quantification in Fluorescence Measurements of Solutions
Complies with the above standards, but is not limited to them.
Features
1. Fluorescence analyzer for measuring fluorescence intensity and spectra.
2. Excitation and emission wavelength scanning for qualitative and quantitative analysis.
3. Suitable for molecular biology, biochemistry, environmental, pharmaceutical and research.
4. Includes light source, monochromators, sample compartment, detector and software.
5. Supports fluorescence measurement for biomolecules, contaminants and material analysis.
FAQ
Q1: What testing scenarios is this analyzer mainly applied to?
A: The fluorescence analyzer is used in molecular biology, biochemistry, environmental monitoring, pharmaceutical research, chemical analysis, food testing and research to measure fluorescence intensity and spectra of samples for qualitative and quantitative analysis of fluorescent compounds, biomolecules and contaminants. It supports biomolecule analysis (protein, nucleic acid labeling and quantification), environmental contaminant detection (PAHs, pesticides), pharmaceutical fluorescence analysis, chemical compound characterization, food safety testing (mycotoxins, vitamins), and research applications. Fluorescence provides high sensitivity and specificity, enabling detection of low-concentration analytes that may not be detectable by absorbance methods. It is essential for molecular biology, biochemistry, environmental, pharmaceutical, chemical and research laboratories requiring fluorescence measurement for biomolecule analysis, contaminant detection, pharmaceutical analysis, chemical characterization and research, where fluorescence provides high sensitivity and specificity for low-concentration analyte detection, supporting molecular biology, environmental monitoring, pharmaceutical development and chemical analysis in research and industrial environments requiring sensitive, selective fluorescence detection and analysis.
Q2: What standards does the analyzer comply with?
A: The fluorescence analyzer complies with relevant IEC 61010 laboratory electrical safety standards and applicable GB/T standards for fluorescence spectrophotometers. Environmental and pharmaceutical applications follow validated methods and good laboratory practice. The instrument complies with relevant electromagnetic compatibility standards. Calibration should use certified fluorescence reference standards (e.g., quinine sulfate) traceable to international standards.
Q3: What is the warranty period for the whole machine and key components?
A: The whole fluorescence analyzer is covered by a 12-month warranty. The light source, monochromators, detector, controller and software are covered, while lamps, cuvettes and calibration standards are consumables or accessories. Damage from improper use or unauthorized repair is excluded. Extended service contracts are available for this analytical instrument.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained analysts familiar with fluorescence spectroscopy, sample handling and data interpretation. We provide operation manuals, training and application support. Proper wavelength selection, blank correction, sample handling, calibration and result interpretation are important for accurate fluorescence analysis.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Wavelength and intensity calibration with certified fluorescence standards recommended every 6-12 months. Routine verification before each analytical batch for regulated work. Light source replacement per usage hours. Full system performance verification annually. Sample compartment and optics cleaned per manufacturer schedule.
Q7: Are supporting consumables universal?
A: Lamps, cuvettes and calibration standards are available from multiple qualified suppliers. Long-term consumable cost is moderate depending on usage and light source type.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes wavelength range, resolution, detector type, sample compartment accessories (microplate, temperature control), software features and automation. Additional accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Standard delivery includes the fluorescence analyzer and accessories with an operation manual. On-site installation, calibration and training are not included for standard orders but can be arranged for analytical laboratory setup. Remote guidance is provided.
Q10: What types of samples are compatible with this equipment?
A: The fluorescence analyzer measures fluorescence of liquid samples in cuvettes (or microplates with accessory) within the wavelength range and intensity specification. Samples must be clear and homogeneous; turbid samples cause scattering and inner filter effects. Highly concentrated samples may exhibit inner filter effects and require dilution. Fluorescent samples should be protected from photobleaching. Users should select appropriate excitation and emission wavelengths for the fluorophore, use matched quartz or glass cuvettes (quartz for UV), perform proper blank correction, calibrate with certified standards, avoid photobleaching by minimizing light exposure, account for inner filter effects at high concentrations, and follow proper sample handling for light-sensitive compounds. The instrument should be allowed proper warm-up time before measurement. For quantitative analysis, proper calibration curves and blank subtraction are essential. The sample compartment should be kept clean, and stray light should be minimized. For environmental or pharmaceutical testing, follow validated methods and include appropriate quality control samples in each analytical batch.
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