Introduction
An inverted fluorescence microscope is a microscope with an inverted optical design, in which the light source and condenser are above the stage and the objectives are below it, combined with fluorescence illumination, and it is designed for the observation of living cells and specimens in culture vessels, such as Petri dishes, flasks, and well plates, and it is a key tool in cell biology, biomedical research, and clinical diagnostics. In the inverted configuration, the specimen is placed on the stage in an open vessel, and the objective lens approaches the specimen from below, allowing the observation of cells at the bottom of the vessel with high magnification, without the need to prepare slides, and this design is ideal for observing adherent and living cells, for cell culture monitoring, and for long-term time-lapse experiments; the fluorescence illumination system, with excitation source, filters, and dichroic mirrors, allows the visualization of fluorescently labeled structures, proteins, and organelles in living cells. Inverted fluorescence microscopes are used in cell biology for studying cell morphology, division, migration, and signaling; in developmental biology for observing embryos and organoids; in cancer research for studying tumor cells; in neuroscience for cultured neurons; in microbiology for biofilm and bacterial studies; in IVF and clinical laboratories; and in drug discovery for cell-based assays, and they can be equipped with digital cameras, incubation chambers for live-cell imaging, and software for image analysis. Features include high-resolution fluorescence imaging, long working distance objectives, and compatibility with culture vessels. With their ability to observe living cells in culture, inverted fluorescence microscopes are essential instruments in the life sciences.
Inverted Fluorescence Microscope LIFM-T2010
Inverted Fluorescence Microscope includes motorized autofocusing mechanism with 0.1μm repeat positioning accuracy for clear imaging. It provides a magnification range of 100× to 400×,...
Inverted Fluorescence Microscope LIFM-T2012
Inverted Fluorescence Microscope offers 10x optical zoom for precise observation in fluorescence imaging applications. Its advanced performance. Features automated stage movement to simp...
Inverted Fluorescence Microscope LIFM-T2011
Inverted Fluorescence Microscope is an advanced Infinite Plan -fluorescent microscope, offers standard magnification range of 40X to 400X. Incorporated with blue and green reflected ligh...
Inverted Fluorescence Microscope LIFM-T2010
Inverted Fluorescence Microscope is an advanced and professional Infinity Plan -fluorescent microscope. It has a standard magnification range from 100 X to 400 X. Offers trinocular head,...
Standards
ISO 9345:2019-Microscopes-Interfacing dimensions for imaging components
Complies with the above standards, but is not limited to them.
Features
1. Inverted fluorescence microscope for observing fluorescently labeled live cells and cultures.
2. Inverted design with objectives below the stage for viewing cells in culture vessels.
3. Suitable for cell biology, live cell imaging, immunology and pharmaceutical research.
4. Multiple fluorescence channels with high-sensitivity detection for live cell observation.
5. Includes light source, filter cubes, objectives, stage, camera port and imaging software.
FAQ
Q1: What testing scenarios is this microscope mainly applied to?
A: The inverted fluorescence microscope is used in cell biology, live cell imaging, immunology, neuroscience, pharmaceutical research, stem cell research and clinical laboratories to observe and image fluorescently labeled live cells, tissue cultures and 3D cultures in their culture vessels. It supports live cell imaging and time-lapse studies, immunofluorescence of cultured cells, cell migration and division observation, protein localization and trafficking studies, drug screening and cytotoxicity assays, stem cell culture monitoring, and 3D cell culture imaging. The inverted design allows viewing through the bottom of culture dishes, flasks and multi-well plates, making it ideal for live cell work. It is essential for cell biology, pharmaceutical and biomedical research laboratories requiring live cell fluorescence imaging for cell dynamics, protein localization, cell behavior studies, drug screening and stem cell research, where the inverted configuration enables observation of cells in their natural culture environment without disturbing them, and fluorescence provides specific molecular visualization in living cells over time in biomedical and pharmaceutical research applications.
Q2: What standards does the microscope comply with?
A: The inverted fluorescence microscope complies with relevant ISO and GB/T standards for optical microscopes, including IEC 60825 for laser safety if laser-equipped. Research applications follow good laboratory practice and applicable institutional biosafety guidelines. The instrument complies with relevant electrical and photobiological safety standards. Filter sets should be matched to the fluorophores used.
Q3: What is the warranty period for the whole machine and key components?
A: The whole microscope is covered by a 12-month warranty. The optical system, objectives, filter cubes, light source and illumination are covered, while bulbs, filters and fuses are consumables excluded from warranty. Damage from improper cleaning, light source mishandling or unauthorized repair is excluded. Extended service contracts are available.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained personnel familiar with fluorescence microscopy, cell culture and live cell imaging. We provide operation manuals, training and application support. Proper filter set selection, cell culture handling, environmental control, exposure management and photobleaching prevention are important for quality live cell imaging.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular maintenance required? What is the recommended calibration cycle?
A: Optical surfaces cleaned regularly with appropriate lens paper and solvent. Light source replacement per usage hours or manufacturer schedule. Filter cube condition and alignment verified periodically. Full optical alignment check recommended annually for high-use instruments. Objective and stage condition verified regularly. Environmental chamber (if equipped) calibration per schedule.
Q7: Are supporting consumables universal?
A: Light bulbs, immersion oil, culture vessels and fluorophore reagents are available from multiple suppliers, though bulb type and filter sets should match the specification. Objectives and filter cubes may be brand-specific. Long-term consumable cost is moderate, with light source replacement being the main recurring item.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes objective set, filter cube configuration, light source type, stage incubator, camera port, motorized stage and software. Additional accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, inverted fluorescence microscopes include on-site installation, alignment and operator training by certified microscopy specialists, particularly for live cell imaging systems with environmental control. Site preparation guidance is provided before delivery.
Q10: What types of samples are compatible with this equipment?
A: The inverted fluorescence microscope observes fluorescently labeled live cells, cultured cells, tissue sections and 3D cultures in compatible culture vessels (dishes, flasks, multi-well plates, slides) within the working distance and magnification range. Samples should be properly labeled with appropriate fluorophores and maintained in suitable culture conditions. Photobleaching and phototoxicity are concerns for live cell imaging. Users should select appropriate filter sets, use proper immersion oil for high-NA objectives, minimize light exposure to prevent photobleaching and phototoxicity, maintain proper environmental conditions (temperature, CO2, humidity) for live cell work, ensure proper sample vessel compatibility with the stage and objectives, and keep optical surfaces clean for optimal image quality.
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