Introduction
A fluorescence microscope is a light microscope that uses fluorescence to visualize specimens, in which the specimen is illuminated with light of a specific wavelength that excites fluorescent molecules, called fluorophores, and the light emitted by these molecules at longer wavelengths is detected to form an image, and it is a powerful tool in biology, medicine, and materials science. In operation, light from an excitation source, such as a mercury, xenon, or LED lamp, passes through an excitation filter that selects the excitation wavelength and a dichroic mirror that reflects this light onto the specimen; the fluorophores in the specimen absorb the excitation light and re-emit light at longer wavelengths, which passes back through the objective, through the dichroic mirror and an emission filter that blocks the excitation light, and to the detector or eyepiece, so that only the fluorescent light forms the image, against a dark background, providing high contrast and specificity. Fluorescence microscopes are used in cell biology for visualizing cellular structures, proteins, and organelles labeled with fluorescent probes; in immunology for immunofluorescence staining; in genetics for fluorescence in situ hybridization (FISH); in microbiology for detecting microorganisms; in neuroscience for tracing neural pathways; and in materials science for studying fluorescent materials, and modern fluorescence microscopes can be equipped with multiple filter sets, digital cameras, and software for image analysis, and confocal and super-resolution systems provide even higher resolution. Features include high sensitivity, specificity, and the ability to observe living specimens. With their power to reveal specific molecular and structural details, fluorescence microscopes are essential instruments in the life sciences.
Fluorescence Microscope LFM-T2011
Fluorescence Microscope is equipped with advanced a trinocular head inclined at 30˚ for comfortable viewing and improves functionality. It features infinite plan objectives ensuring con...
Fluorescence Microscope LFM-T2010
Fluorescence Microscope has a widefield infinity optical system for clear and precise imaging. Its 30-degree inclined ocular tubes ensure comfort even during extended periods of use. The...
Fluorescence microscope LFM-T2010
Fluorescence microscope is an advanced optical instrument designed for studying specimens at the microscopic level. Modular design enhances the compatibility of biological and fluorescen...
Fluorescence Microscope LFM-T2010
Fluorescence Microscope is equipped with advanced a WF10x/22mm eyepiece and a quintuple nosepiece. Its infinity plan objective ensures clear and detailed imaging. The optional water obje...
Fluorescence Microscope LFM-T2010
Fluorescence Microscope features infinity plan objectives from 4x to 100x, delivering flat, high-contrast images for accurate observation. Its trinocular head ensures comfortable viewing...
Fluorescence Microscope LFM-T2020
Fluorescence Microscope has design of Siedentopf type trinocular viewing head (dual light transform) inclined at 30°, 360° rotation, and interpupillary distance of 48 to76 mm. This Mic...
Fluorescence Microscope LFM-T2011
Fluorescence microscope is equipped with advanced epi-fluorescence system, comes in siedentopf binocular head design. Its rack and pinion mechanism, useful in coarse and fine focusing of...
Fluorescence Microscope LFM-T2010
Fluorescence microscope is equipped with advanced epi-fluorescence system, comes in binocular and trinocular head design. This fluorescence microscope offers high quality performance. It...
Standards
ISO 9345:2019-Microscopes-Interfacing dimensions for imaging components
Complies with the above standards, but is not limited to them.
Features
1. Fluorescence microscope for observing fluorescently labeled specimens and samples.
2. High-intensity light source with filter sets for excitation and emission of fluorophores.
3. Suitable for cell biology, immunology, pathology, neuroscience and research laboratories.
4. Supports multiple fluorescence channels for simultaneous multi-color imaging.
5. Includes light source, filter cubes, objectives, eyepieces, camera port and software.
FAQ
Q1: What testing scenarios is this microscope mainly applied to?
A: The fluorescence microscope is used in cell biology, immunology, pathology, neuroscience, molecular biology, pharmaceutical research and clinical laboratories to observe and image fluorescently labeled cells, tissues, microorganisms and molecules. It supports immunofluorescence staining visualization, cell structure and protein localization studies, live cell imaging, pathology specimen examination, neuronal tracing, FISH (fluorescence in situ hybridization), and drug discovery imaging. The fluorescence technique provides high sensitivity and specificity for visualizing specific molecules, structures or processes within cells and tissues. It is essential for biological and medical research laboratories, clinical pathology departments, pharmaceutical companies and academic institutions requiring fluorescence imaging for cell biology research, protein localization, immunological studies, pathological diagnosis, neuroscience research and drug development, where fluorescence microscopy enables visualization of specific molecules and structures with high contrast and specificity that cannot be achieved by brightfield microscopy alone in life science and biomedical applications.
Q2: What standards does the microscope comply with?
A: The fluorescence microscope complies with relevant ISO and GB/T standards for optical microscopes, including IEC 60825 for laser safety if laser-equipped. Clinical diagnostic applications follow validated laboratory procedures and applicable quality management standards including ISO 15189 where accredited. The instrument complies with relevant electrical and photobiological safety standards. Filter sets should be matched to the fluorophores used.
Q3: What is the warranty period for the whole machine and key components?
A: The whole microscope is covered by a 12-month warranty. The optical system, objectives, filter cubes, light source and illumination are covered, while bulbs, filters and fuses are consumables excluded from warranty. Damage from improper cleaning, light source mishandling or unauthorized repair is excluded. Extended service contracts are available.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained personnel familiar with fluorescence microscopy, fluorophore properties and sample handling. We provide operation manuals, training and application support. Proper filter set selection, light source alignment, exposure control, sample preparation and photobleaching management are important for quality fluorescence imaging.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular maintenance required? What is the recommended calibration cycle?
A: Optical surfaces cleaned regularly with appropriate lens paper and solvent. Light source (mercury, metal halide or LED) replacement per usage hours or manufacturer schedule. Filter cube condition and alignment verified periodically. Full optical alignment check recommended annually for high-use instruments. Objective and eyepiece condition verified regularly. Fluorescence intensity reference standards can be used for performance monitoring.
Q7: Are supporting consumables universal?
A: Light bulbs, immersion oil, slides and fluorophore reagents are available from multiple suppliers, though bulb type and filter sets should match the specification. Objectives and filter cubes may be brand-specific. Long-term consumable cost is moderate, with light source replacement being the main recurring item.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes objective set, filter cube configuration, light source type (mercury, LED, laser), camera port, stage and software. Additional objectives, filter sets and accessories can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Standard delivery includes the microscope, objectives, filter cubes and accessories with an operation manual. On-site installation, alignment and training are recommended for fluorescence microscopes and can be arranged at additional cost. Remote guidance is provided.
Q10: What types of samples are compatible with this equipment?
A: The fluorescence microscope observes fluorescently labeled cells, tissues, microorganisms and molecules within the magnification and filter set capabilities. Samples should be properly prepared with appropriate fluorophores, mounting media and coverslips. Unlabeled samples will not produce fluorescence signal. Photobleaching is a concern for sensitive fluorophores and live cell imaging. Users should select appropriate filter sets for the fluorophores, use proper immersion oil for high-NA objectives, minimize light exposure to prevent photobleaching, ensure proper sample mounting and sealing, follow darkroom or low-light conditions for fluorescence observation, and keep optical surfaces clean for optimal image quality and signal intensity.
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