Introduction
A fluorescence spectrophotometer is an analytical instrument used to measure the fluorescence of the samples, which provides the information about the concentration and the properties of the fluorescent compounds, and it is used in the chemistry, the biology, the pharmaceutical, the environmental, and the many other applications. The fluorescence spectrophotometer excites the sample with the light at the excitation wavelength, and the sample emits the fluorescence at the longer wavelengths, which is measured by the detector, providing the fluorescence spectrum and the intensity; the fluorescence spectrophotometer consists of an excitation light source, such as the xenon lamp, the excitation monochromator, a sample holder, the emission monochromator, a detector, and the software, and it provides features such as the wavelength scanning and the quantitative analysis; the fluorescence spectrophotometer is used for the analysis of the fluorescent compounds, for the measurement of the concentrations, for the study of the molecular interactions, for the analysis of the pharmaceuticals, for the environmental analysis, for the research, and for many other applications, and it is an essential instrument for the fluorescence analysis. Fluorescence spectrophotometers are used in the chemistry laboratories, in the biology laboratories, in the pharmaceutical industry, in the environmental laboratories, and for many other applications. With their fluorescence measurement, fluorescence spectrophotometers are essential for the sensitive analysis.
Fluorescence Spectrophotometer LFS-T2013
Fluorescence Spectrophotometer features a wavelength range of 200 to 900 nm allowing detection of a wide variety of fluorescent materials. It offers variable scanning speeds within the t...
Fluorescence Spectrophotometer LFS-T2014
Fluorescence Spectrophotometer features a xenon lamp with an excitation wavelength of 365nm. With 17 selectable levels of gain adjustment, it offers flexibility in emission spectrum scan...
Fluorescence Spectrophotometer LFS-T2011
Fluorescence Spectrophotometer is a high performance equipment with a 150 W Xenon wavelength accuracy to meet most of the measurements. Quantum yield is also available for this fluoresce...
Fluorescence Spectrophotometer LFS-T2012
Fluorescence Spectrophotometer consist of a high quality 150 W Xenon light source and photoelectric multiplier tube detectors for providing sufficient light intensity signal to detect se...
Fluorescence Spectrophotometer LFS-T2010
Fluorescence Spectrophotometer consist of a high quality 150 W Xenon light source and photoelectric multiplier tube detectors for providing sufficient light intensity signal to detect se...
Standards
European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible
ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers
ASTM E579-20-Standard Test Method for Limit of Detection and Limit of Quantification in Fluorescence Measurements of Solutions
Complies with the above standards, but is not limited to them.
Features
1. Fluorescence spectrophotometer for measuring fluorescence emission and excitation spectra.
2. High-sensitivity detection with xenon lamp and monochromators for spectral analysis.
3. Suitable for analytical chemistry, life sciences, pharmaceutical and environmental research.
4. Measures fluorescence intensity, quantum yield, kinetics and polarization.
5. Includes light source, monochromators, sample chamber, detector and analysis software.
FAQ
Q1: What testing scenarios is this spectrophotometer mainly applied to?
A: The fluorescence spectrophotometer is used in analytical chemistry, life sciences, pharmaceutical research, environmental monitoring, materials science and clinical research to measure fluorescence emission and excitation spectra of fluorescent compounds and materials. It supports quantitative analysis of fluorescent analytes, protein and nucleic acid characterization, drug discovery and development, environmental pollutant detection, material fluorescence properties, fluorescence kinetics and binding studies. The high-sensitivity detection enables measurement of low-concentration fluorescent species with excellent signal-to-noise ratio. It is essential for research laboratories, pharmaceutical companies, academic institutions and analytical facilities requiring sensitive fluorescence measurement for compound characterization, biomolecular interaction studies, drug discovery, environmental analysis, materials research and clinical research, where fluorescence provides highly sensitive, specific detection of molecules, interactions and processes that cannot be easily measured by absorbance or other techniques in chemical, biological and materials science applications.
Q2: What standards does the spectrophotometer comply with?
A: The fluorescence spectrophotometer supports methods referenced in ASTM E579 for fluorescence measurement, ISO standards for fluorescence spectroscopy, and relevant GB/T standards. The hardware complies with IEC 61010 laboratory safety standards. Calibration should use certified fluorescence reference standards traceable to international standards. Wavelength and intensity calibration should be performed regularly.
Q3: What is the warranty period for the whole machine and key components?
A: The whole spectrophotometer is covered by a 12-month warranty. The light source, monochromators, detector, controller and software are covered, while xenon lamp, cuvettes and standards are consumables excluded from warranty. Damage from improper use or unauthorized repair is excluded. Extended service contracts are available for this high-value instrument.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires specialist training in fluorescence spectroscopy, sample preparation and data analysis. We provide comprehensive training, operation manuals and application support. Proper wavelength and intensity calibration, sample handling, parameter optimization and result interpretation are essential for quality fluorescence data.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Wavelength calibration with certified reference standards recommended every 3-6 months. Intensity calibration with fluorescence standards periodically. Xenon lamp replacement per usage hours. Full system performance verification annually. Sample chamber and optics cleaned per manufacturer schedule. Routine verification with a standard before critical measurements.
Q7: Are supporting consumables universal?
A: Xenon lamps, cuvettes and calibration standards are available from specialized suppliers, though instrument-specific parts should be verified. Long-term consumable cost is moderate, with lamp replacement being the main recurring item.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes detector type, monochromator configuration, sample chamber accessories, temperature control, polarization accessory and software modules. Custom orders require detailed application specification and extend lead-time. Users should define sample types, required sensitivity, measurement modes and environmental conditions before configuration.
Q9: Does the purchase include on-site installation and commissioning service?
A: Yes, fluorescence spectrophotometers include on-site installation, commissioning, calibration and comprehensive operator training by certified application specialists. Site preparation guidance is provided before delivery to ensure vibration isolation, temperature control, power and environmental requirements are met.
Q10: What types of samples are compatible with this equipment?
A: The fluorescence spectrophotometer analyzes liquid, solid and gas samples with fluorescent properties within the wavelength range and detection limits. Non-fluorescent samples may require derivatization with fluorescent labels. Highly concentrated samples may show inner filter effects and require dilution. Photobleaching-sensitive samples require careful measurement parameters. Users should select appropriate excitation and emission wavelengths, use matched fluorescence cuvettes, calibrate wavelength and intensity regularly, account for inner filter effects and scattering, control sample temperature for reproducible results, and follow proper lamp warm-up and instrument stabilization procedures for accurate, sensitive fluorescence measurements.
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