Digital inverted microscope

Introduction

A digital inverted microscope is a microscope with an inverted design, in which the objective lenses are below the stage, and a digital camera, used for the observation and the imaging of the samples in the culture vessels, the microplates, and the large specimens, and it is used in the cell biology, the microbiology, the clinical, and the research laboratories. The digital inverted microscope consists of an inverted stand with the objective lenses below the stage, a stage that holds the culture vessels or the plates, an illumination system above the stage, a digital camera, and a computer with the imaging software, and it provides the observation of the samples from below, which is suitable for the observation of the cells in the culture flasks and the plates, and the digital camera captures the images and the videos, which are displayed and analyzed on the computer; the digital inverted microscope is used for the cell culture monitoring, for the observation of the living cells, for the phase contrast and the fluorescence microscopy, for the observation of the specimens in the plates, for the documentation, for the image analysis, and for many other applications, and it provides the digital imaging with features such as the adjustable magnification, the camera control, and the image analysis software; the digital inverted microscope is an essential instrument in the cell biology laboratory. Digital inverted microscopes are used in the cell biology and the microbiology laboratories, in the clinical laboratories, in the pharmaceutical industry, in the research institutions, and for many other applications. With their digital imaging, digital inverted microscopes are essential for the observation and the documentation of the samples.

Digital Inverted Microscope LIDM-T2011

Digital Inverted Microscope is designed to deliver magnification within a range of 40x to 400x. It integrates a 4K camera with an adjustable screen, enabling the capture and recording of...

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Digital Inverted Microscope LIDM-T2010

Digital Inverted Microscope is an advanced optical microscope designed to deliver 40x to 400x magnification. It integrates a tablet camera for high-quality image analysis enabling real-...

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Standards

ISO 9345:2019-Microscopes-Interfacing dimensions for imaging components

Complies with the above standards, but is not limited to them.

Features

1. Digital inverted microscope for live cell observation from below with integrated camera.

2. Inverted microscope with objectives under stage, LED illumination, phase contrast and digital camera.

3. Suitable for cell culture, live cell imaging, microbiology, hematology and biomedical research.

4. Includes body, objectives (4x/10x/20x/40x), CMOS camera, software, power cord and manual.

5. Supports brightfield, phase contrast and optional fluorescence of cells in flasks, plates and dishes.

FAQ

Q1: What testing scenarios is this microscope mainly applied to?

A: The digital inverted microscope views live cells from below the stage (objectives under stage point up through vessel bottom), ideal for cell culture monitoring (mammalian cell lines, stem cells - check confluency, morphology, contamination without opening vessels), live cell imaging (time-lapse division/migration/apoptosis, wound healing), cell counting/viability (hemocytometer, trypan blue), microbiology (bacteria/yeast counting, motility), hematology (blood smears, cell counting), drug screening (96-well plate cytotoxicity), and immunofluorescence. Phase contrast is standard for unstained live cells; fluorescence (GFP, antibodies) optional. Typical objectives 4x (scan/confluency), 10x, 20x, 40x (detail). Camera 2-20 MP CMOS/sCMOS.

Q2: What standards does the microscope comply with?

A: Complies with IEC 61010 (electrical safety), IEC 61326 (EMC), ISO 15223 (medical devices if clinical), and GB/T standards. For clinical/diagnostic use (hematology, pathology), follow medical device regulations (FDA, CE/MDR, ISO 13485, ISO 15189). For GLP/GMP, maintain IQ/OQ, calibration records (camera, illumination, magnification with stage micrometer), SOPs. For fluorescence, follow light safety (IEC 60825 if laser; LED interlocks). Digital images stored with metadata (magnification, scale bar, date) for reproducibility.

Q3: What is the warranty period for the whole microscope and key components?

A: Whole microscope covered 12-24 months (some 3 years for body). LED light source (20,000-50,000 h, long life), mercury lamp if fluorescence (200-300 h, wear), camera sensor (5-10 years), objectives (10+ years if cared for), fluorescence filters (fade with UV) are consumables/accessories. Damage from objective scratches/fungus, liquid spills on optics, wrong immersion oil, camera UV damage, or unauthorized repair is excluded. Extended warranty and annual optics service available. Durable, low-maintenance if optics protected.

Q4: Is this device difficult to operate? Is professional training required?

A: Basic use simple: turn on, place culture vessel on stage (cells on bottom facing objectives), select objective, focus (coarse/fine up through vessel bottom), align phase contrast (condenser annulus matches objective phase ring via Bertrand lens), capture image via software. Life science personnel with cell culture experience can operate; training 1-2 days provided (microscopy basics, camera/software, phase alignment). Maintenance: clean stage daily, clean objectives weekly with lens paper + solvent, professional optics service annually. Safety: biological (cell cultures BSL-1/2, disinfect spills, biohazard waste), chemical (immersion oil, stains, fixatives - PPE, fume hood for formaldehyde), light (fluorescence UV/blue - do not look directly, mercury lamp high pressure/UV - cool before replacing). For long-term live cell imaging use environmental chamber (37 C, 5% CO2, humidity).

Q5: What is the typical lead-time for order delivery?

A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.

Q6: Is regular calibration required? What is the recommended calibration cycle?

A: Calibrate/verify every 6-12 months: objective magnification with stage micrometer (verify scale bar per objective), camera (flat-field, resolution, color balance), illumination intensity, focus stability. Routine: phase ring alignment when objectives changed, image quality check before each use. For GLP/GMP/clinical, maintain records and IQ/OQ. For quantitative analysis (cell size, counting), calibrate pixels-per-micron for each objective. Fluorescence mercury lamp replace at 200-300 h and realign. LED sources stable (50,000 h) but verify annually.

Q7: Are supporting consumables universal?

A: Objectives brand-specific (RMS thread mostly standard but parfocal distance varies - use OEM/certified; phase objectives must match condenser). Immersion oil universal (Cargille, Olympus; use non-toxic for live cell). Lens paper/cleaner universal. Fluorescence filters specific to fluorophore and microscope (Chroma, Semrock, OEM). Hemocytometers universal. Long-term cost: immersion oil, lens paper, occasional mercury lamp (if fluorescence), objective replacement if damaged ($500-5000 each).

Q8: Can I customize parameters or supporting accessories?

A: Customization includes objectives (4x/10x/20x/40x/100x oil, long working distance for plastic), contrast (brightfield, phase contrast, DIC, fluorescence, darkfield), camera (CMOS 2-20 MP, sCMOS low-light for fluorescence, color/monochrome), software (basic capture vs advanced: cell counting, confluency, time-lapse, z-stack, 3D, 21 CFR Part 11), environmental control (stage-top incubator 37 C/5% CO2/humidity), motorized stage/autofocus for multi-well scanning. Accessories: extra objectives, immersion oil, stage micrometer, hemocytometer, fluorescence filter cubes (GFP/RFP/DAPI), environmental chamber, dust cover, anti-vibration table, IQ/OQ/PQ, certifications (CE, ISO 9001, IEC 61010, medical device if clinical).

Q9: Does the purchase include on-site installation and commissioning service?

A: Standard delivery includes microscope, objectives, condenser, camera, software, power cord, manual (immersion oil, stage micrometer, fluorescence accessories not included). On-site installation/training typically included or recommended (camera setup, phase alignment, Kohler illumination, software; manufacturer/certified technician standard, especially for fluorescence/motorized). Always align phase contrast and calibrate scale with stage micrometer before use. Basic brightfield/phase models self-install feasible but training recommended.

Q10: What types of samples are compatible with this equipment?

A: Compatible: live cell cultures in flasks/plates/dishes (cells on bottom surface), suspended cells in hemocytometer, blood smears, microorganisms (bacteria, yeast, fungi) in wet mounts, fixed/stained cells on slides, embryos (IVF), small organisms (C. elegans, zebrafish larvae). Not compatible: thick/opaque samples (use stereomicroscope or section), thick vessels beyond objective working distance (use long-working-distance objective), 100x oil through plastic (use glass-bottom vessels), samples requiring nanoscale detail (use SEM/TEM), or fluorescence without fluorescence module. For 100x oil/high NA use glass-bottom dishes. For live cell long imaging use environmental chamber.