Introduction
A thermal cycler is a laboratory instrument used to perform the polymerase chain reaction (PCR), which amplifies specific DNA sequences by cycling the temperature of the reaction mixture through repeated heating and cooling steps, and it is an essential instrument in molecular biology, genetics, diagnostics, and biotechnology. The thermal cycler consists of a block that holds reaction tubes or plates, a heating and cooling system, typically based on Peltier elements, and a controller that precisely regulates the temperature of the block according to a programmed profile; the PCR reaction is cycled through denaturation, typically at about 95 degrees Celsius, in which the double-stranded DNA separates; annealing, typically at 50-65 degrees Celsius, in which the primers bind to the target sequence; and extension, typically at 72 degrees Celsius, in which the DNA polymerase synthesizes new DNA, and each cycle doubles the amount of target DNA, so that after many cycles, the target sequence is amplified millions of times. Thermal cyclers are used for PCR amplification in gene cloning, DNA sequencing, genotyping, mutation analysis, pathogen detection, forensic DNA profiling, and many other applications, and they are available in various configurations, including standard block cyclers, gradient cyclers that allow the optimization of annealing temperatures, and instruments with multiple blocks for high throughput, with features such as programmable temperature profiles, touch screens, and data management. With their central role in PCR, thermal cyclers are essential instruments in molecular biology and diagnostics.
Mini Thermal Cycler LMTHC-T2010
Mini Thermal Cycler offers a compact, space-efficient design with a temperature setting range of 0 to 100℃. It is equipped with advanced a 5-inch color touch screen that offers intuitiv...
Gradient Thermal Cycler LGTM-T2010
Gradient Thermal Cycler offers a temperature setting range of 4℃ to 105℃ and a gradient temperature range of 30 to 105℃. It is equipped with advanced an advanced semiconductor Peltier...
Mini Gradient Thermal Cycler LMTC-T2015
Mini Gradient Thermal Cycler accommodates 16 × 0.2 mL tubes, providing an efficient and space-saving solution for high-performance in limited sample volumes. It features advanced, custo...
Mini Gradient Thermal Cycler LMTC-T2014
Mini Gradient Thermal Cycler features a capacity of 32 × 0.2 mL tubes, making it ideal for small-scale reactions and efficient thermal cycling. Enables rapid temperature changes, ensuri...
Touch Thermal Cycler (Gradient) LTCG-T2026
Touch Thermal Cycler (Gradient) features a high-capacity block that accommodates 384 wells of 0.4 mL each for high-throughput applications. It uses semiconductor technology for rapid the...
Touch Thermal Cycler (Gradient) LTCG-T2025
Touch Thermal Cycler (Gradient) features a block capacity of 96 × 0.2 mL tubes, for high-throughput workflows in research and diagnostics. The system displays a gradient temperature dis...
Touch Thermal Cycler (Gradient) LTCG-T2024
Touch Thermal Cycler (Gradient) features a block capacity of 96 × 0.2 mL, ideal for high-throughput applications in standard 96-well plates. Offers both block and individual tube temper...
Touch Thermal Cycler (Gradient) LTCG-T2015
Touch Thermal Cycler (Gradient) features gradient temperature setting range of 30℃ to 105℃ for optimal efficiency. Equipped with 7-inch touchscreen display for intuitive navigation and...
Touch Thermal Cycler (Gradient) LTCG-T2014
Touch Thermal Cycler (Gradient) offers gradient range of 1℃ to 42℃ enabling precise optimization of annealing temperatures. Its advanced Peltier technology assures uniform and stable t...
Touch Thermal Cycler (Basic) LTCB-T2014
Touch Thermal Cycler (Basic) offers heating range of 4℃ to 105℃, ensuring control for optimization. Its intuitive touchscreen interface enables user-friendly operation with quick progr...
Touch Thermal Cycler (Gradient) LTCG-T2023
Touch Thermal Cycler (Gradient) operates within a temperature range of 0 to 105℃, ensuring precise thermal cycling for applications. Three independent gradient blocks enable simultaneou...
Mini Gradient Thermal Cycler LMTC-T2012
Mini Gradient Thermal Cycler is designed to hold 96 × 0.2 ml plates and 8 × 0.2 ml tube strips, making it ideal for applications. It uses advanced Peltier technology for high performan...
Mini Gradient Thermal Cycler LMTC-T2013
Mini Gradient Thermal Cycler has a dual-block capacity for double 48×0.2 ml microtubes and 8×0.2 ml tube strips. It achieves the fastest heating rate of 6°C/sec, ensuring optimal temp...
Mini Gradient Thermal Cycler LMTC-T2011
Mini Gradient Thermal Cycler has a larger capacity, supporting 96×0.2ml plates, 12×8×0.2ml strips, and 0.2ml tubes. It offers a slightly faster cooling rate of 4.5°C/sec, ensuring ef...
Mini Gradient Thermal Cycler LMTC-T2010
Mini Gradient Thermal Cycler provides a capacity for 32×0.2 ml microtubes and 4×8×0.2 ml tube strips, ensuring versatility in sample handling. It boasts a maximum heating rate of 5°C...
Touch Thermal Cycler (Gradient) LTCG-T2013
Touch Thermal Cycler (Gradient) is highly compatible with standard tubes, 8-well strips, and 96-well plates. It features a user-friendly interface with a graphical menu and color touchsc...
Advanced Thermal Cycler (Gradient) LATG-T2010
Advanced Thermal Cycler (Gradient) accommodates 96 tubes of 0.2 ml capacity for efficient processing of multiple samples. It utilizes 8 Peltier elements to achieve precise and effective ...
Touch Thermal Cycler (Gradient) LTCG-T2022
Touch Thermal Cycler (Gradient) with customized 6 pcs long service life peltier heating units. With 5°C/s maximum Ramping rate, this cycler saves operation time of user. It has 3 × (32...
Touch Thermal Cycler (Basic) LTCB-T2013
Touch Thermal Cycler (Basic) with customized 6 pcs long service life peltier heating units. With 5°C/s maximum Ramping rate, this cycler saves operation time of user. It has 3 × (32 ×...
Advanced Thermal Cycler (Gradient) LATG-T2013
Advanced Thermal Cycler (Gradient) comes with 30 to 99.9℃ gradient temperature range, advanced peltier based semi-conductor technology. It uses 6 pieces of advanced (US) Marlow peltier ...
Advanced Thermal Cycler (Gradient) LATG-T2012
Advanced Thermal Cycler (Gradient) comes with 30 to 99.9℃ gradient temperature range, advanced peltier based semi-conductor technology. It uses 6 pieces of advanced (US) Marlow peltier ...
Advanced Thermal Cycler (Gradient) LATG-T2011
Advanced Thermal Cycler (Gradient) comes with 30 to 105℃ gradient temperature range, advanced peltier based semi-conductor technology. It uses 6 pieces of advanced (US) Marlow peltier w...
Advanced Thermal Cycler (Basic) LATB-T2012
Advanced Thermal Cycler (Basic) is adopted with advanced peltier based semi-conductor technology for thermal uniformity, is used in multiple based experiments. It uses 6 pieces of advanc...
Advanced Thermal Cycler (Basic) LATB-T2011
Advanced Thermal Cycler (Basic) is adopted with advanced peltier based semi-conductor technology for thermal uniformity, is used in multiple based experiments. It uses 6 pieces of advanc...
Advanced Thermal Cycler (Basic) LATB-T2010
Advanced Thermal Cycler (Basic) is adopted with advanced peltier based semi-conductor technology for thermal uniformity, is used in multiple based experiments. It uses 6 pieces of advanc...
Touch Thermal Cycler (Gradient) LTCG-T2012
Touch Thermal Cycler (Gradient) with customized 8 pcs long service life Marlow (US) peltier heating units. With 5°C/s maximum ramping rate, this cycler saves operation time of user. Its...
Touch Thermal Cycler (Gradient) LTCG-T2021
Touch Thermal Cycler (Gradient) with customized 8 pcs long service life Marlow (US) peltier heating units, allows double block gradient function by forming 4 circuits to control 4 temper...
Touch Thermal Cycler (Gradient) LTCG-T2020
Touch Thermal Cycler (Gradient) with customized 8 pcs long service life Marlow (US) peltier heating units, allows double block gradient function by forming 4 circuits to control 4 temper...
Touch Thermal Cycler (Gradient) LTCG-T2011
Touch Thermal Cycler (Gradient) with customized 8 pcs long service life Marlow (US) peltier heating units. With 5°C/s maximum ramping rate, this cycler saves operation time of user. Its...
Touch Thermal Cycler (Gradient) LTCG-T2010
Touch Thermal Cycler (Gradient) with customized 8 pcs long service life Marlow (US) peltier heating units. With 5°C/s maximum ramping rate, this cycler saves operation time of user. Its...
Touch Thermal Cycler (Basic) LTCB-T2012
Touch Thermal Cycler (Basic) comes with customized 8 pcs long service life Marlow (US) peltier heating units. Each thermal block has 4 independent temperature control sensors. With 5°C/...
Touch Thermal Cycler (Basic) LTCB-T2011
Touch Thermal Cycler (Basic) comes with customized 8 pcs long service life Marlow (US) peltier heating units. Each thermal block has 4 independent temperature control sensors. With 5°C/...
Touch Thermal Cycler (Basic) LTCB-T2010
Touch Thermal Cycler (Basic) comes with customized 8 pcs long service life Marlow (US) peltier heating units. Each thermal block has 4 independent temperature control sensors. With 5°C/...
Standards
ISO 20836:2021-Microbiology of the food chain-PCR for the detection of microorganisms-Thermal performance testing of thermal cyclers
Complies with the above standards, but is not limited to them.
Features
1. Thermal cycler for polymerase chain reaction (PCR) nucleic acid amplification.
2. Precise temperature control with rapid ramp rates for efficient amplification.
3. Suitable for molecular biology, clinical diagnostics, forensics, research and education.
4. Includes sample block, heating/cooling system, controller, software and operation manual.
5. Supports DNA amplification, genotyping, cloning and molecular biology research.
FAQ
Q1: What testing scenarios is this cycler mainly applied to?
A: The thermal cycler is used in molecular biology, clinical diagnostics, forensic science, pharmaceutical research, agricultural biotechnology, food safety and educational institutions to perform polymerase chain reaction (PCR) for nucleic acid amplification, genotyping, cloning and molecular biology research. It supports DNA amplification for genetic analysis, genotyping and SNP detection, gene cloning and recombinant DNA work, clinical diagnostic PCR assays, forensic DNA profiling, GMO detection in food, pathogen detection, and educational molecular biology experiments. The thermal cycler provides precise, rapid temperature cycling through denaturation, annealing and extension steps, enabling exponential amplification of target DNA sequences. It is essential for molecular biology, clinical, forensic, agricultural, food and educational laboratories requiring PCR amplification for genetic analysis, genotyping, cloning, diagnostics, forensics, food safety and research, where precise temperature cycling enables exponential nucleic acid amplification supporting a wide range of molecular biology applications from basic research to clinical diagnostics, forensic identification and agricultural biotechnology, requiring reliable, accurate thermal cycling for reproducible PCR results.
Q2: What standards does the cycler comply with?
A: The thermal cycler complies with relevant IEC 61010 laboratory electrical safety standards and applicable GB/T standards for molecular biology equipment. Clinical diagnostic applications follow validated laboratory procedures, applicable medical device regulations and quality management standards including ISO 15189 where accredited. The instrument complies with relevant electromagnetic compatibility standards. For diagnostic use, assays should be validated per applicable regulations.
Q3: What is the warranty period for the whole machine and key components?
A: The whole thermal cycler is covered by a 12-month warranty. The sample block, heating/cooling system, controller and housing are covered, while sample tubes, fuses and accessories are consumables or accessories. Damage from improper use, sample spillage or unauthorized repair is excluded. Extended service contracts are available for this laboratory instrument.
Q4: Is this device difficult to operate? Is professional training required?
A: Operation requires trained molecular biology personnel familiar with PCR principles, assay design and thermal cycling. We provide operation manuals, training and application support. Proper reaction setup, primer design, cycle programming, block selection, contamination prevention and result interpretation are essential for successful PCR.
Q5: What is the typical lead-time for order delivery?
A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.
Q6: Is regular calibration required? What is the recommended calibration cycle?
A: Temperature calibration and block uniformity verification recommended every 6-12 months. Sample block condition checked periodically. Heating/cooling performance verified. Routine decontamination recommended, particularly for clinical and forensic use. Software updates installed as available. Full system performance verification annually.
Q7: Are supporting consumables universal?
A: PCR tubes, plates, seals, reagents, enzymes and primers are available from multiple molecular biology suppliers, though tube/plate compatibility with the block should be verified. Long-term consumable cost depends on experiment volume and reagent choices.
Q8: Can I customize parameters or supporting accessories?
A: Customization includes block format (96-well, 384-well, gradient), ramp rate, temperature range, control features, connectivity and software. Additional accessories and consumables can be ordered separately. Custom orders extend delivery lead-time.
Q9: Does the purchase include on-site installation and commissioning service?
A: Standard delivery includes the thermal cycler and accessories with an operation manual. On-site installation, calibration and training are not included for standard orders but can be arranged for laboratory setup. Remote guidance is provided.
Q10: What types of samples are compatible with this equipment?
A: The thermal cycler amplifies DNA and cDNA samples in compatible tubes or plates within the block format and temperature specification. RNA requires reverse transcription before PCR (RT-PCR). Different block formats require specific tube/plate types. Samples should be properly prepared nucleic acids or crude samples with validated protocols. Users should follow proper PCR setup including master mix preparation, primer design, template concentration, cycle parameters, use appropriate tubes/plates for the block, prevent contamination with dedicated pre- and post-PCR areas, include appropriate controls (NTC, positive controls), and follow good molecular biology practices. For clinical diagnostic use, assays should be validated and performed per established SOPs in a quality-controlled environment. The block should be allowed proper cooling between runs, and heated lid temperature should be set appropriately to prevent condensation. Gradient cyclers enable optimization of annealing temperature across the block.
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