Spectrophotometer

Introduction

A spectrophotometer is an analytical instrument used to measure the amount of light absorbed or transmitted by a sample as a function of wavelength, and it is one of the most widely used instruments in chemistry, biochemistry, biology, and many other fields, for quantitative and qualitative analysis. In operation, light from a source is passed through a monochromator, which selects a specific wavelength, and the light of that wavelength passes through the sample in a cuvette; the instrument measures the intensity of the light transmitted through the sample relative to the incident light, and calculates the absorbance, which is related to the concentration of the absorbing species by the Beer-Lambert law, and by scanning the wavelength, the instrument produces an absorption spectrum that can be used to identify substances and to determine concentrations. Spectrophotometers are available in various types, including UV-Vis spectrophotometers for the ultraviolet and visible regions, visible spectrophotometers, and infrared and near-infrared instruments, and they can be single-beam or double-beam designs, with features such as wavelength scanning, automatic measurement, and data analysis software. They are used for quantifying analytes in solutions, for determining the concentration of proteins, nucleic acids, and other biomolecules, for measuring the color of products, for monitoring reactions and kinetics, for purity analysis, for clinical diagnostics, for water and environmental analysis, for food and beverage quality control, and for pharmaceutical analysis, in research, quality control, clinical, and industrial laboratories. With their versatility and accuracy, spectrophotometers are essential analytical instruments in virtually every laboratory.

Portable Spectrophotometer LSP-T2030

Portable Spectrophotometer is adopted with 45/0 geometry (45 ring shaped illumination, 0 observe degree), where the standard white board is included in the optical system for great accur...

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Standards

European Pharmacopoeia 2.2.25-Spectrophotometry, ultraviolet and visible

ASTM E275-08(2022)-Standard Practice for Describing and Measuring Performance of Ultraviolet, Visible, and Near-Infrared Spectrophotometers

Complies with the above standards, but is not limited to them.

Features

1. Spectrophotometer for measuring absorbance and transmittance of samples across wavelengths.

2. UV-Visible or visible range for qualitative and quantitative spectroscopic analysis.

3. Suitable for chemical, biological, pharmaceutical, environmental, food and research labs.

4. Includes light source, monochromator, sample compartment, detector and software.

5. Supports concentration measurement, spectral scanning, kinetics and method development.

FAQ

Q1: What testing scenarios is this spectrophotometer mainly applied to?

A: The spectrophotometer is used in chemical analysis, biological research, pharmaceutical quality control, environmental monitoring, food testing, clinical diagnostics and research laboratories to measure the absorbance or transmittance of samples at specific wavelengths for qualitative and quantitative analysis. It supports chemical compound concentration determination, nucleic acid and protein quantification in molecular biology, pharmaceutical content and impurity testing, environmental pollutant analysis, food additive and nutrient measurement, clinical diagnostic assays, and spectral scanning for compound identification. The spectrophotometer is one of the most widely used analytical instruments, providing fast, accurate measurement based on Beer-Lambert law. It is essential for chemical, biological, pharmaceutical, environmental, food and clinical laboratories requiring absorbance measurement for quantitative analysis, compound identification, quality control, kinetic studies and research, where spectrophotometric measurement provides sensitive, specific, reproducible analysis of compounds and samples across a wide range of applications in analytical chemistry, life sciences, pharmaceuticals, environmental monitoring, food safety and clinical diagnostics, serving as a foundational analytical tool in laboratory science.

Q2: What standards does the spectrophotometer comply with?

A: The spectrophotometer supports methods referenced in USP, EP and ChP monographs, ASTM E169 for UV-Vis quantitative analysis, ISO standards, and relevant GB/T standards. The hardware complies with IEC 61010 laboratory safety standards. Electronic records may be configured for 21 CFR Part 11 compliance. Wavelength and absorbance accuracy should be verified with certified reference materials (e.g., holmium oxide, didymium filters).

Q3: What is the warranty period for the whole machine and key components?

A: The whole spectrophotometer is covered by a 12-month warranty. The light source, monochromator, detector, controller and software are covered, while lamps, cuvettes and calibration standards are consumables excluded from warranty. Damage from improper use or unauthorized repair is excluded. Extended service contracts are available.

Q4: Is this device difficult to operate? Is professional training required?

A: Operation requires trained analysts familiar with spectrophotometric principles, sample handling and data interpretation. We provide operation manuals, training and application support. Proper wavelength selection, blank correction, sample handling, calibration and result interpretation are important for accurate spectrophotometric analysis.

Q5: What is the typical lead-time for order delivery?

A: Standard models require 7-30 working days. In-stock units can ship within 3-7 working days. Custom configurations or large built-to-order systems may require additional time, and we confirm the exact schedule before order confirmation.

Q6: Is regular calibration required? What is the recommended calibration cycle?

A: Wavelength and absorbance calibration with certified reference materials recommended every 6-12 months. Routine verification before each analytical batch for regulated work. Light source replacement per usage hours. Full system performance verification annually. Sample compartment and optics cleaned per manufacturer schedule.

Q7: Are supporting consumables universal?

A: Lamps, cuvettes and calibration standards are available from multiple qualified suppliers. Long-term consumable cost is moderate depending on usage and light source type.

Q8: Can I customize parameters or supporting accessories?

A: Customization includes wavelength range (UV-Vis, Vis), resolution, detector type, sample compartment accessories, software features and automation. Additional accessories can be ordered separately. Custom orders extend delivery lead-time.

Q9: Does the purchase include on-site installation and commissioning service?

A: Standard delivery includes the spectrophotometer and accessories with an operation manual. On-site installation, calibration and training are not included for standard orders but can be arranged for quality control laboratory setup. Remote guidance is provided.

Q10: What types of samples are compatible with this equipment?

A: The spectrophotometer analyzes liquid samples in cuvettes within the wavelength and absorbance range. Samples must be clear and homogeneous; turbid samples cause scattering errors. Highly corrosive samples require compatible cuvette material. Very high absorbance samples should be diluted to the linear range (typically 0.1-1.0 AU). Users should select appropriate wavelength for the analyte, use matched cuvettes, perform proper blank correction, calibrate with certified standards, allow lamp warm-up, verify wavelength accuracy regularly, and follow Beer-Lambert law principles for quantitative analysis. For solid or powder samples, appropriate accessories (integrating sphere, solid sample holder) may be required. The instrument should be allowed proper warm-up time before measurement for stable results.